The High Pure Viral RNA Kit is for general laboratory use and purifies viral RNA from a variety of samples such as serum and plasma.

• Remove inhibitors that interfere with downstream assays, ensuring greater assay specificity, sensitivity, and reproducibility. 
• Prepare RNA samples in only 20 minutes.
• Obtain concentrated RNA that is suitable for downstream applications.
• Minimize RNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade RNA.
• Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.

Contents

• Binding Buffer
• Poly (A), lyophilizate
• Inhibitor Removal Buffer
• Wash Buffer
• Elution Buffer
• High Pure Spin Filter Tubes 

• Collection Tubes

Figure 1: RT-PCR analysis of MS2 RNA isolated with the High Pure Viral RNA Kit. 

Serial dilutions of purified MS2 RNA were applied to High Pure filter tubes, processed according to the kit protocol, and eluted in a total volume of 50 µL. An aliquot (3.5 µL) of each eluate was analyzed by two-step RT-PCR, using primers that produced a 961 bp product. Products were analyzed by agarose gel electrophoresis. For each sample listed below, the "number of molecules per PCR" assumes that all the RNA applied to the High Pure tube was recovered in the eluate.
 

Lane 1: 3.5 x 107 molecules/RT-PCR
Lane 2: 3.5 x 105 molecules/RT-PCR
Lane 3: 3.5 x 103 molecules/RT-PCR
Lane 4: 3.5 x 102 molecules/RT-PCR
Lane 5: 3.5 x 101 molecules/RT-PCR
Lane M: DNA Molecular Weight Marker III

Result: Even when the template contained a theoretical maximum of 35 molecules viral RNA, RT-PCR generated a product that was detectable on an agarose gel.

Research Use Only

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