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◆Storage:Store at -20℃ for one year. Avoid repeated freeze.
◆Storage Buffer:PBS with 0.02% sodium azide, 100 μg/mL BSA and 50% glycerol.
◆Prodoct Information:
Protein full name | Actin, cytoplasmic 1 |
Synonyms | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse β-Actin |
Uniprot ID | P60710 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 45 kDa |
◆Applications:
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | spleen, kidney, brain |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 2000 | colon, kidney, liver |
ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549 |
◆Background:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
◆NOTE:
1. This product is intended for research only.
2. This product is recommended to dilute with the Primary Antibody Dilution Buffer (Cat:P322).
◆Images:
Western blot analysis of beta Actin(Cat: SA2194)at dilution of 1: 1000
Western blot analysis of beta Actin(Cat: SA2194)at dilution of 1: 1000
Immunohistochemistry of paraffin embedded human colon using actin (Cat: SA2194) at dilution of 1: 1000 (200x lens)
Immunohistochemistry of paraffin embedded rat liver using actin (Cat: SA2194) at dilution of 1: 1000 (200x lens)
Immunohistochemistry of paraffin embedded mouse stomach using actin (Cat: SA2194) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded rat kidney using actin (Cat: SA2194) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded human liver cancer using actin (Cat: SA2194) at dilution of 1: 1000 (200x lens)
Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (Cat: SA2194) at dilution of 1: 1000
Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (Cat: SA2194) at dilution of 1: 1000
品牌: | 爱普科学 |
规格: | 100uL |
性状: | 液体 |
储存温度: | -20℃ |
保质期: | 12个月 |
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